Fig. 7

P150^Glued deficiency enhances tubulin acetylation in spinal motor neurons. a-b Western blot analysis shows the expression of acetylated (ac-), tyrosinated (tyro-), detyrosinated (detyro-) and total alpha tubulin (TUBA) in the spinal cord homogenate of 9-month-old Dctn1^LoxP/LoxP and Dctn1^LoxP/LoxP; Thy1-Cre mice. Actin was used as loading control. Bar graphs show the quantification of ac-TUBA, tyro-TUBA and detyro-TUBA level normalized with total TUBA (n = 8 per genotype). Data were presented as mean ± SEM, ^***p < 0.001. Unpaired t-test was used for statistical analysis. c, d Representative images show p150^Glued (green), ac-TUBA (red) and CHAT (blue) staining in the lumbar spinal cord of 9-month-old Dctn1^LoxP/LoxP (c) and Dctn1^LoxP/LoxP; Thy1-Cre (d) mice. Four mice per genotype and 10 sections per mouse were examined. Arrows point to CHAT-positive ventral roots with weak ac-TUBA staining in Dctn1^LoxP/LoxP mice and substantial ac-TUBA staining in Dctn1^LoxP/LoxP; Thy1-Cre mice. Scale bars: 500 μm (low-magnification images), 20 μm (high-magnification images)