Fig. 5
Quantification of GFAP-stained cells in the hippocampus of male Wistar rats fed a control (NF), HF45 or HF60 diet from the age of two months until the age of six months, evaluated by immunohistochemistry. Food in HF groups was offered in isocaloric amounts to the NF group (control group). a Number of GFAP positive cells in the NF, HF45 and HF60 groups. GFAP-stained cells were counted from the whole glia present in the hippocampus samples, and referred to the glia area (positive cells/mm2). Data represent mean ± SD (n = 6 in control and HF60 groups; n = 5 in HF45 group). Statistics: P value indicates statistical trend towards significance (Student’s t-test). b An enlarged GFAP positive cell is shown as example of the specificity of the antibody. c Representative hippocampus sections immunostained for GFAP in the NF and HF60 groups. Insets: enlargement of the corresponding framed areas. *indicates GFAP-positive cells. Abbreviations: CA: cornu ammonis, G: glia. Scale bar: 200 μm and 50 μm for insets