Fig. 2
The disease-associated mutations reduce TREM2 binding to oAβ1–42. a Schematic representation of NHD and AD-associated sTREM2 variants tagged with Fc. b Immunoblotting of sTREM2-Fc in the conditioned medium or cell lysate from HEK 293 T cells expressing AD-associated TREM2 mutations (R47H, R62H, D87N and T96 K) or NHD-associated TREM2 mutations (Y38C and T66 M). (c and d) Bar graphs show the quantification of Western blots from panel B (n = 3, one-way ANOVA). e The purified Fc, wild-type sTREM2 WT-Fc (WT) and sTREM2-Fc with disease-associated mutations were analyzed by silver staining. (f and g) Solid phase binding assay showing the binding affinity of oAβ1–42 to WT and different variants of TREM2-Fc (n = 3, two-way ANOVA). Data information: Data represent mean ± SD. ***, p < 0.001