Fig. 3

GSK-3β activation in the retina. a Representative Western blots of protein extracts from WT and rd10 retinas (at P19 and P21). b Densitometric analysis of several membranes as those shown in a. Levels of phosphorylated Akt and GSK-3β were normalized to those of total Akt and GSK-3β, respectively. Results represent the mean + SEM. n = 4–5, *p ≤ 0.05 (unpaired 2-tailed Student’s t test). c Representative images of P21 retinal sections from WT and rd10 mice, immunostained for pAkt^Ser473 and pGSK-3β^Ser9 (green). Nuclei are stained with DAPI (blue). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 60 μm