Fig. 8
Acute inflammation induces Tregs after adoptive transfer of tolerogenic BMDCs. GM-CSF-generated BMDCs were cultured in media, harvested, and 1.5 × 106 in 0.25 mls were adoptively transferred i.v. at each of 2 weekly transfers and the frequencies of splenic CD4+ cells and CD4+CD25+Foxp3+ Tregs were evaluated by flow cytometric analysis. Gates were drawn to include > 98% of the total lymphocytes or > 98% of CD4+ T cells. a Splenic T cell frequencies were determined seven days after the second transfer of BMDCs to naïve recipients. Control mice received no BMDCs. Means ± SEM were determined for control (n = 11) and BMDC-transferred mice (n = 17) and were evaluated by Student’s two-tailed t-test whereby p ≤ 0.05 compared to acontrol mice. b After the second transfer of BMDCs, mice were intoxicated with four 16 mg/kg doses of MPTP, MPTP alone, or PBS alone. T cell frequencies were determined two days after intoxication. Means ± SEM were determined for n = 5 mice per each group and evaluated by one-way ANOVA followed by Tukey’s post hoc test whereby p ≤ 0.05 compared to mice treated with aPBS alone or bMPTP alone