Fig. 3

AMPA treatment results in potent, long-lasting decreases in ISF Aβ levels that slowly recover. a APP/PS1 mice (n = 5) were treated with 5 μM AMPA using reverse microdialysis for 8 h resulting in a decrease in ISF Aβ levels of 32.7 ± 3.0% from baseline. After 8 h, AMPA was removed from the microdialysis perfusion buffer. Aβ levels continued to decline for 3 h post-treatment to reach a maximum reduction of 56.7 ± 1.7% from baseline. For the next 40 h, ISF Aβ levels gradually increased. When the experiment was ended at 52 h, ISF Aβ levels had increased 23.5 ± 3.0% to reach 64.8 ± 3.0% of basal levels, which was a significant increase from the lowest Aβ levels post-treatment (p = 0.0245, two-way ANOVA, Sidak post hoc test). b APP/PS1 mice (n = 3) were treated with 5 μM AMPA followed by co-treatment with AMPA and 100 μM NBQX for 14 h. The addition of NBQX did not alter the decrease in Aβ levels caused by AMPA treatment (one-way ANOVA, Sidak post hoc test). c 5 μM AMPA was infused by rev md into APP/PS1 mice for a 30-min period, after which the perfusion buffer was changed to artificial CSF for 24 h. AMPA treatment caused a 41.30 ± 9.45% decrease in ISF Aβ levels in the 22–24 h after 30-min dosage (n = 3, p = 0.035, two-tailed t-test). Data plotted as mean ± SEM