Fig. 4

Comparison of proteomic profiles of LPS-induced pro-inflammatory and 5xFAD AD pathological microglial states. a Scatter plot representation of relative expression (log2 transformed fold change) of all proteins differentially expressed in both LPS-treated WT (vs. untreated WT) as well as 5xFAD (vs WT) mouse models. Overall Pearson’s correlation coefficient was 0.47, p < 0.001. b Heat map representing proteins with concordant changes in expression in microglia isolated from LPS-induced pro-inflammatory and 5xFAD neurodegeneration mouse models (compared to untreated WT microglia). c Heat map representing protein expression changes seen only in 5xFAD microglia but not in LPS-treated WT microglia. d Comparison of proteomic profiles of microglia from advanced aged mice with microglia derived from acute neuroinflammatory and 5xFAD neurodegeneration mouse models. Ageing-associated proteins and their relative expression data were obtained from an existing microglial proteome from young (age 3–4 mo) and aged (age 20–24 mo) WT C57BL/6 J mice [10]. Aging-associated proteins that were differentially expressed in either LPS-treated WT (vs WT) or 5xFAD (vs WT) conditions were included and an unsupervised hierarchical clustering analysis was performed to identify 4 distinct clusters. Grey boxes indicate proteins that were not significantly altered in the respective comparison