Fig. 1

MHCI depletion affects the production and the infiltration of CD8⁺ T cells in mSOD1 mice. a Schematic representation C57BL6.129P2-B2mtm1Unc/J females bred with C57BL/6JSOD1^G93A males mice in order to obtain transgenic mice null for β2microglobulin. b Representative FACS scatter plots of CD3⁺/CD8⁺ T cells in the peripheral blood of NTG+/+ mice, G93A+/+, NTG−/− and G93A−/− mice at 123 d. c Longitudinal FACS measurement of the percentage of CD3⁺/CD8⁺ T cells in the peripheral blood of G93A+/+; G93A−/− mice and relative controls at 70, 123 and 140 d. Data are reported as the mean ± SEM of six independent experiments (6 mice) for G93A+/+ and NTG+/+ mice and eight independent experiments (8 mice) for G93A−/− and NTG−/− at each time point. ^****P < 0.0001 (G93A+/+ vs NTG+/+); ^°°°°P < 0.0001 (NTG−/− vs NTG+/+ and G93A+/+); ^####P < 0.0001 (G93A−/− vs NTG+/+ and G93A+/+). d Real-time PCR for the CD8α receptor transcript in the lumbar and cervical spinal cord of G93A+/+, G93A−/− mice compared to NTG +/+ littermates at 123 and 140 d. Data are normalized to β-actin and expressed as the mean ± SEM fold change ratio between G93A+/+ mice, G93A−/− mice and control mice from four independent experiments for each genotype at both stages. ^**P < 0.05; ^****P < 0.0001 (G93A+/+ vs G93A−/−); ^°P < 0.05; ^°°P < 0.01; (G93A−/− or G93A+/+ vs NTG) by one-way ANOVA with Tukey’s post-analysis