Fig. 7

MHCI and CTLs depletion accelerate hindlimb muscle atrophy in SOD1 mutant mice but delays that of triceps brachii muscles in SOD1 mutant mice. (a) Representative images of the gastrocnemius, tibialis anterior and triceps brachii muscles showing increased muscle atrophy of hindlimbs muscles in G93A-/- mice at 123 d, but less weight loss of triceps brachii at 140 d compared to G93A+/+ mice; Bar, 0.5 cm. (b) Muscle wasting was calculated by measuring of the gastrocnemius and tibialis anterior muscle weight of G93A-/- and G93A+/+ mice compared to relative NTG littermates (NTG+/+; NTG-/-). At 123 d, six, eight, nine and nine GC muscles and six, eight, eight and ten TA muscles were analyzed for NTG+/+, NTG-/-, G93A+/+ and G93A-/- mice, respectively. At 140 d, 11, 14, ten and 16 GC muscles and 11, 14, 16 and 16 TA muscles were analyzed for NTG+/+, NTG-/-, G93A+/+ and G93A-/- mice, respectively. Percent muscle atrophy in (c) was calculated relative to NTG mice. (d) Triceps brachii muscle wasting was calculated by measurement of the muscle weight of G93A+/+ and G93A-/- mice compared to relative NTG littermates (NTG+/+; NTG-/-) at 140 d. Six, seven, ten and ten independent experiments were analyzed for NTG+/+, NTG-/-, G93A+/+ and G93A-/-, respectively. The percentage of muscle atrophy in (e) was calculated relative to corresponding NTG mice. Data are presented as mean ± SEM of three independent experiments for G93A+/+ mice and four independent experiments for G93A-/- mice. ^*P < 0.05; ^**P < 0. 01 (G93A+/+ vs G93A-/); ^°°°°P < 0.0001