Fig. 2
From: PU.1 regulates Alzheimer’s disease-associated genes in primary human microglia
Microarray analysis of PU.1 silencing. a Heatmap generation of the top 180 differentially expressed genes (log2 fold change > 1.5, adjusted p-value < 0.001) with PU.1 siRNA versus control siRNA in mixed glial cultures. Corresponding changes in pericyte-only cultures are shown. b, c Venn diagrams revealing 51 upregulated genes by PU.1 silencing, 26 of which were specific to mixed glial cultures and 129 downregulated genes, 122 of which were specific to mixed glial cultures. d, e Volcano plots displaying log2 fold change versus –log adjusted p-value for all genes (grey) and genes of interest displaying significant reduction (dark red), mild reductions (light red), no change (black), mild induction (light green), or significant induction (dark green) in mixed glial cultures or pericyte only cultures. f Validation of selected genes by qRT-PCR (n = 3 independent microglial cultures) in mixed glial cultures transfected with 50 nM PU.1 siRNA versus control siRNA for 7 days, colour coded by their significance from microarray analysis. Data is displayed as fold change of mRNA genes in PU.1 siRNA treated cultures relative to control siRNA samples as determined by the 2^-ΔΔCt method (g) Log2 fold changes between microarray and qRT-PCR analysis demonstrated good correlation (R2 = 0.75)