Fig. 1
From: Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis
Generation of Nrf1 expression and conditional Nfr1 alleles. (a) Genomic structure of Nrf1, the targeting construct, the targeted Nrf1LacZ and Nrf1flox alleles, and the deleted allele. Exons are indicated as E1-E12. The gray and black bars indicate the regions used in the targeting construct. A black arrow indicates the translational start site for the Nrf1 protein. Red arrows indicate PCR primers used for PCR genotyping of the wildtype and floxed alleles. Red boxes indicate loxP sites, and green boxes indicate FRT sites. (b) PCR genotyping using U5 and U3 primers for wildtype (203 bp) and floxed (317 bp) alleles. (c, d) Nrf1 expression during retinogenesis revealed by LacZ expression in Nrf1LacZ/+ retinas at E13.5 (c) and P20 (d). (e) Schematic representation of the developing retinal cells expressing Six3 and rhodopsin. Proliferative retinal progenitor cells expressing Six3 will give rise to all mature retinal cells. Rhodopsin is expressed in differentiated rod photoreceptor cells. (f–i) Nrf1 expression detected by immunofluorescent staining on E13.5 wildtype (f) and Nrf1f/f;Six3-Cre (g) retinal sections and on 6-week-old wildtype (h) and Nrf1f/f;Rho-iCre (i) retinal sections. Scale bars: 100 μm in C, 50 μm in d-i. ONL: outer nuclear layer. INL: inner nuclear layer. GCL: ganglion cell layer. WT: wildtype. NBL: neural blast layer