Table 1 Primer sets used for qRT-PCR
From: Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis
Gene | Position | 5′-sequence-3’ |
|---|---|---|
b-actin | Forward | CAACGGCTCCGGCATGTGC |
Reverse | CTCTTGCTCTGGGCCTCG | |
CCND1 | Forward | GCACTTTTGGTCAGCTAGCT |
Reverse | GACATGGCCCTAAACCTTCT | |
Gli1 | Forward | ACTGGGGTGAGTTCCCTTCT |
Reverse | AGGACTACCCAGCAAATCCT | |
Mapt | Forward | AATGGAAGACCATGCTGGAG |
Reverse | TCCCAATCTGAGTCCCAAAG | |
Ret | Forward | TGGCACACCTCTGCTCTATG |
Reverse | CTGTTCCCAGGAACTGTGGT | |
Stmn3 | Forward | CCCGAACACCATCTACCAGT |
Reverse | CTTCTGCAGCTCTTCCAAGG | |
Ncan | Forward | GTGGCTGCTTCTCCTAGTGG |
Reverse | AATGTCTCGCAGGGAGCTTA | |
Ina | Forward | TTCGGGAATACCAGGACTTG |
Reverse | GTGCTAAACCGCGTCTCTTC | |
Islr2 | Forward | CTCTGCCTTTTCAAGGATGC |
Reverse | CGCTGAGTTGAAAGGCCTAC | |
Nell2 | Forward | CACAGTTGACCTTTCCTGCT |
Reverse | CAGCACAAATGGCCATTCTT | |
Stmn2 | Forward | GCAATGGCCTACAAGGAAAA |
Reverse | GGTGGCTTCAAGATCAGCTC | |
Gap43 | Forward | GTGCTGCTAAAGCTACCACT |
Reverse | CTTCAGAGTGGAGCTGAGAA | |
Nrn1 | Forward | CCAGGGGAATGACTTCAAGA |
Reverse | TTTCGCTTTTCTGGAGGAGA | |
Syt4 | Forward | TGTTGTAGGTGATGGTTTCA |
Reverse | AGACCATGGTTCTTAGGTGA | |
Dcx | Forward | ACAGATGTCAACCGGGAAAG |
Reverse | TCGTTCGTCAAAATGTCCAA | |
Pou4f1 | Forward | AGGCCTATTTGCCGTACAA |
Reverse | CGTCTCACACCCTCCTCAGT | |
Irx4 | Forward | GAGACCACCAGCACACTGAA |
Reverse | AGGTGGAAACCTGTGTGAGG | |
Cx3cr1 | Forward | AGCCCAGGGGAAGAAATAGA |
Reverse | CTCTGTTGGCTCCAGTCTCC | |
Capn3 | Forward | GCTTCTGGAGGAAGACGATG |
Reverse | TTTGGGAACCTCGTAGATGG | |
Igfbp7 | Forward | GGAAAATCTGGCCATTCAGA |
Reverse | TGCGTGGCACTCATACTCTC | |
Vit | Forward | GCGTCTACGCGTCTTACTCC |
Reverse | CCCTTTTGGGGCTTACTTTC | |
Nid1 | Forward | ACCATCACCTTCCAGGAGTG |
Reverse | GCATAGCGCAAGATCCTCTC | |
Stab1 | Forward | ACAAGATCTTCAGCCGCCTA |
Reverse | AGTTTGTCACGGTGGTCCTC | |
Spp1 | Forward | TGCACCCAGATCCTATAGCC |
Reverse | CTCCATCGTCATCATCATCG | |
Tgfbi | Forward | GGATGTCCTGAAGGGAGACA |
Reverse | ATTGGTGGGAGCAAAAACAG | |
Cox4i2 | Forward | AGCTGAGCCAAGCAGAGAAG |
Reverse | GCCCATCACTGTCTTCCATT | |
Idh1 | Forward | AGGTTCTGTGGTGGAGATGC |
Reverse | GACGCCCACGTTGTATTTCT | |
Dna2 | Forward | CGAAGTTCTGTGCATCCTGA |
Reverse | TTCTCAGACACCGAATGCTG | |
Gdap1 | Forward | CTGTGAGGCCACTCAGATCA |
Reverse | TGAGCTCAGGATGCAAAATG | |
Shmt2 | Forward | CTCTTTGCTTCGGACCACTC |
Reverse | TTCTCCCTCTGCAGAAGCTC | |
Cpt1a | Forward | CCAGGCTACAGTGGGACATT |
Reverse | AAGGAATGCAGGTCCACATC | |
Sardh | Forward | ACTCGGTTGTCTTCCCACAC |
Reverse | CCTGTCGCTCTTGAAACACA | |
Ucp2 | Forward | GCCACTTCACTTCTGCCTTC |
Reverse | GAAGGCATGAACCCCTTGTA | |
Pmaip1 | Forward | CCCAGATTGGGGACCTTAGT |
Reverse | AGTTATGTCCGGTGCACTCC | |
Rab32 | Forward | CTCTTCTCCCAGCACTACCG |
Reverse | CAAATGCTCCAAGAGCTTCC | |
Ldha | Forward | AGGCTCCCCAGAACAAGATT |
Reverse | TCTCGCCCTTGAGTTTGTCT | |
HK1 | Forward | GAAGCCAAATGGGACTGTGT |
Reverse | CACGCACAGATTGGTTATGC | |
Pfkp | Forward | GAAGCCAAATGGGACTGTGT |
Reverse | CACGCACAGATTGGTTATGC | |
Tpi1 | Forward | CCTGGCCTATGAACCTGTGT |
Reverse | CAGGTTGCTCCAGTCACAGA | |
Pgam2 | Forward | AGGAGCTGCCTACCTGTGAA |
Reverse | GGGCTGCAATAAGCACTCTC | |
Mfn1 | Forward | GCTGTCAGAGCCCATCTTTC |
Reverse | CAGCCCACTGTTTTCCAAAT | |
Mfn2 | Forward | GTCCTGGACGTCAAAGGGTA |
Reverse | GCAGAACTTTGTCCCAGA | |
Opa1 | Forward | GATGACACGCTCTCCAGTGA |
Reverse | TCGGGGCTAACAGTACAACC |