Table 1 Demographics and clinical characteristics of ALS (A,B,C) and healthy control (D) individuals included in the discovery proteomics (A, B) and in the re-test immunoassays (C, D)
A. DISCOVERY EXPERIMENT: ALS PLASMA SAMPLES | |||||||||
ALS type | ALSFRS-R early | ALSFRS-R late | Gender M/F | Age at onset (years) | PRL | Time from onset to death or last visit (months) | Time from onset to first sample (months) | Time between first and last sample (months) | Site of disease onset limb/ bubar |
Slow (n = 6) | 43 (41–45) | 31.5 (27–35) | 5 M / 1F | 58.1 (35–71) | 0.25 (0.1–0.4) | 107 (97, 254) | 76 (30–192) | 58 (48–69) | 4 limb /2 bulbar |
Fast (n = 6) | 43.8 (41–46) | 28.6 (22–35) | 3 M / 3F | 61.3 (48–67) | 1.5 (1–1.9) | 20.5 (11–32) | 10.2 (5–19) | 13.8 (7–22) | 3 limb /3 bulbar |
B. DISCOVERY EXPERIMENT: ALS PBMC SAMPLE TO FORM THE REFERENCE POOL | |||||||||
ALS Pool | ALSRS-R | Gender M/F | Age at onset (years) | PRL | Time from onset to death or last visit (months) | Time from onset to sampling (months) | Site of disease onset limb/ bubar | ||
Slow(3) Fast (2) n = 5 | 31 (22–42) | 3 M /2 F | 65.8 (57–68) | 0.8 (0.02–2.5) | 64.6 (8–120) | 19 (7–44) | 3 limb /2 bulbar | ||
C. RE-TEST EXPERIMENT: ALS PLASMA SAMPLES | |||||||||
ALS type | ALSRS-R | Gender M/F | Age at onset (years) | PRL | Time from onset to death or last visit (months) | Time from onset to sampling (months) | Site of disease onset limb/ bubar | ||
Slow (n = 23) | 39 (18–47) | 18 M / 5F | 65.25 (37–86.9) | 0.216 (0.03–0.47) | 120.4 (28–335.2) | 62.8 (7–248.8) | 15 limb /8 bulbar | ||
Fast (n = 24) | 33 (13–43) | 8 M / 16F | 62.8 (34.8–82.4) | 1.48 (0.74–3.6) | 28.1 (5–50) | 14.8 (2.8–29.8) | 13 limb /11 bulbar | ||
D. RE-TEST EXPERIMENT: HEALTHY CONTROLS PLASMA SAMPLES | |||||||||
n = 29 | Gender M/F | Age at sampling (years) | |||||||
8 M / 17F | 60.9 (50.8–73) | ||||||||