Fig. 2
From: Differences in neurotropism and neurotoxicity among retrograde viral tracers
Multi-trans-synaptic retrograde tracing via injection of RV-B2C-EGFP and PRV-152 into the lymph nodes. (a-d) RV-B2C-EGFP retrogradely labeled M1 (A), S1 (A), VPM (B), LPAG (C), and cerebellum (D), but not PVN, LC, or Bar when injected into the lymph nodes. (e-h) Retrograde labeling by PRV-152 from the lymph nodes was observed in M1 (E), S1 (E), PVN (F), LPAG (G), LC and Bar (H), but not VPM (F) and cerebellum (H). (i) The Map of inputs to LHA labeled by RV-B2C-EGFP (upper line) and PRV-152 (lower line) at coronal view are arranged along the rostrocaudal direction. Retrogradely labeled neurons are indicated as colored dots. Numbers indicate position of sections relative to bregma (mm). (j) The semi-quantified labeling discrepancy between RV-B2C-EGFP and PRV-152 in the whole brain. Three mice were analyzed for each viral tracer. Insets show magnified images of the boxed regions. Scale bars = 1 mm in lower magnification images; 100 μm in higher magnification insets