Fig. 7
From: Differences in neurotropism and neurotoxicity among retrograde viral tracers
Gene profiling analysis of rAAV2-retro-labeled and RV-∆G-labeled neurons via single-cell RNA sequencing. (a) Flow chart of single-cell isolation and RNA sequencing. (b) Gene cluster analysis of the neuronal groups infected by rAAV2-retro-Cre-tagBFP and RV-∆G-EGFP. (c) Expression of neuronal marker genes including VLGUT1, VGLUT2, GAD65, GAD67, TH, ChAT, and Pet1 in the rAAV2-retro-Cre-tagBFP and RV-∆G-EGFP groups. (d) Expression heat-map of potential receptor candidates for rabies virus, including Ncam, Chrna, and Ngfr, in each sample from rAAV2-retro and RV-∆G labeled groups. (e) These are no significant difference of Ncam1 expression between rAAV2-retro and RV-∆G labeled groups, P=0.088. Chrna, nicotinic acetylcholine receptor gene; Ncam, neural cell adhesion molecule gene; Ngfr: Nerve growth factor receptor