Fig. 11

Aβo-induced suppression of LTP in the hippocampus is abolished by mimics of miRNAs enriched in NSC-exo. a) Schematic of the experimental design. MicroRNAs (scrambled or mimics of miR-322, miR17 and miR-485) or PBS (vehicle) were injected ICV into adult mice 24 h before euthanasia. Schaffer collateral field recording of LTP (indicated as percent of baseline in the slope of fEPSPs) was performed on brain slices in the presence of Aβ oligomers. Control mice were injected with PBS (vehicle). Aβo abolished LTP in PBS treated mice and in scrambled-treated mice but not in miRNAs combo-treated mice (b). c) The fEPSP amplitude for the final 10 min (time points 50–60 min post high frequency stimulation) were averaged for each condition. Aβ oligomers significantly reduced LTP in brain slices from mice injected with vehicle or with scrambled miRNA, but not in brain slices from mice treated with combo miRNAs mimics. N = 6 mice/group (2 slices per mouse). *p < 0.05 Student’s two-tailed T-test