Fig. 6

PKG activation counteracts LTP impairment in oTau-treated slices. a Perfusion for 10 min with 8-pCPT-cGMP (1 μM) before LTP induction rescued the oTau-induced LTP reduction (n = 10; ANOVA for repeated measures: F_(1,19) = 24.037, p < 0.0001 compared with slices perfused with oTau). Perfusion with oTau 20 min prior to tetanization diminished LTP (oTau: n = 10; vehicle: n = 11; F_(1,19) = 26.310, p = 0.0001 comparing oTau vs. vehicle) while 8-pCPT-cGMP alone did not affect potentiation (n = 11; F_(1,20) = 0.100, p = 0.755, compared with vehicle). b Quantification of the residual potentiation at 30 and 120 min from LTP curves shown in A (Vehicle: n = 11 slices/11 animals, 5 males and 6 females; oTau: n = 10 slices/9 animals, 5 males and 4 females; 8-pCPT-cGMP: 11 slices/10 animals, 5 males and 5 females; 8-pCPT-cGMP + oTau: 10 slices/10 animals, 5 males and 5 females). One-way ANOVA among all: F_(3,38) = 8.496, p < 0.0001 at 30 min and F_(3,38) = 9.964, p < 0.0001 at 120 min; Bonferroni’s: p = 0.001 and p < 0.0001 between oTau and Vehicle or oTau + 8-pCPT-cGMP at 30 min and 120 min, respectively. *** p < 0.005, ****p < 0.0001