Fig. 9
Hepatocyte-derived apoE does not affect apoE or Aβ levels in the brain. Cortical tissues were sequentially homogenized in PBS and 5 M guanidine HCl buffer. Aβ and apoE levels were quantified by ELISA. a Levels of PBS-soluble apoE in cortex of male mice. There were no significant effect of apoE isoform (F2,50 = 3.040, p = 0.568), Cre expression (F1,50 = 0.3673, p = 0.5472) or interaction (F2,50 = 0.1696, p = 0.8445). (n; APP/PS1/E2F: 8 Cre−/−,12 Cre+/−; APP/PS1/E3F: Cre−/−,12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 8 Cre+/−) b Levels of PBS-soluble apoE in cortex of female mice. There was no significant effect of apoE isoform (F2,42 = 0.1669, p = 0.8468), Cre expression (F1,42 = 0.02187, p = 0.8831) or interaction (F2,42 = 2.277, p = 0.1151). (n; APP/PS1/E2F: 5 Cre−/−, 8 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 6 Cre+/−; APP/PS1/E4F: 11 Cre−/−, 7 Cre+/−) c Levels of PBS-soluble Aβ40 in the cortex of male mice. There was a significant effect of apoE isoform (F2,49 = 10.12, p = 0.0002), but not Cre expression (F1,49 = 0.002707, p = 0.9587) or interaction (F2,49 = 0.4113, p = 0.6650). (n; APP/PS1/E2F: 8 Cre−/−, 12 Cre+/−; APP/PS1/E3F: 10 Cre−/−, 12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 8 Cre+/−) d Levels of PBS-soluble Aβ40 in the cortex of female mice. There was a significant effect of apoE isoform (F2,42 = 3.495, p = 0.0394), but not Cre expression (F1,42 = 0.4314, p = 0.5149) or interaction (F2,42 = 1.217, p = 0.3065). (n; APP/PS1/E2F: 8 Cre−/−, 12 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 8 Cre+/−) e Levels of PBS-soluble Aβ42 in cortex of male mice. There was a significant effect of apoE isoform (F2,50 = 5.328, p = 0.0080), but not Cre expression (F1,50 = 0.07241, p = 0.7890), or interaction (F2,50 = 0.1759, p = 0.8392). (n; APP/PS1/E2F: 8 Cre−/−, 12 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 8 Cre+/−) f Levels of PBS-soluble Aβ42 in the cortex of female mice. There was a trend towards a significant effect of apoE isoform (F2,42 = 2.597, p = 0.0864), and no significant effect of Cre expression (F1,42 = 0.1586, p = 0.6925) or interaction (F2,42 = 0.2245, p = 0.7999). (n; APP/PS1/E2F: 5 Cre−/−, 8 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 6 Cre+/−; APP/PS1/E4F: 11 Cre−/−, 7 Cre+/−) g Levels of guanidine-soluble apoE in the cortex of male mice. There was a trend towards a significant effect of apoE isoform (F2,50 = 2.513, p = 0.0912), and no effect of Cre expression (F1,50 = 0.8026, p = 0.3746) or interaction (F2,50 = 0.9035, p = 0.4116). (n; APP/PS1/E2F: 8 Cre−/−, 12 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 8 Cre+/−) h Levels of guanidine soluble apoE in the cortex of female mice. There was a significant effect of apoE isoform (F2,42 = 3.669, p = 0.0340), but no significant effect of Cre expression (F1,42 = 0.0003, p = 0.9857) or interaction (F2,42 = 0.2874, p = 0.7517). (n; APP/PS1/E2F: 5 Cre−/−, 8 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 6 Cre+/−; APP/PS1/E4F: 11 Cre−/−, 7 Cre+/−) i Levels of guanidine-soluble Aβ40 in the cortex of male mice. There was a significant effect of apoE isoform (F2,48 = 7.273, p = 0.0017), but not Cre expression (F1,48 = 0.1102, p = 0.7414) or interaction (F2,48 = 2.333, p = 0.1079). (n; APP/PS1/E2F: 7 Cre−/−, 12 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 7 Cre+/−) j Levels of guanidine-soluble Aβ40 in the cortex of female mice. There was a significant effect of apoE isoform (F2,41 = 9.575, p = 0.0004), but no significant effect of Cre expression (F1,41 = 0.1030, p = 0.7499) and no interaction (F2,41 = 2.354, p = 0.1077). (n; APP/PS1/E2F: 4 Cre−/−, 8 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 6 Cre+/−; APP/PS1/E4F: 11 Cre−/−, 7 Cre+/−) k Levels of guanidine-soluble Aβ42 in the cortex of male mice. There was a significant effect of apoE isoform (F2,49 = 10.88, p = 0.0001), but no significant effect of Cre expression (F1,49 = 0.05731, p = 0.8118), and a trend towards a significant interaction (F2,49 = 2.925, p = 0.0631). (n; APP/PS1/E2F: 7 Cre−/−, 12 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 12 Cre+/−; APP/PS1/E4F: 5 Cre−/−, 8 Cre+/−). l Levels of guanidine-soluble Aβ42 in the cortex of female mice. There was a significant effect of apoE isoform (F2,41 = 16.32, p < 0.0001), but not Cre expression (F1,41 = 0.4266, p = 0.5173) and no interaction (F2,41 = 1.722, p = 0.1914). (n; APP/PS1/E2F: 4 Cre−/−, 8 Cre+/−; APP/PS1/E3F: 11 Cre−/−, 6 Cre+/−; APP/PS1/E4F: 11 Cre−/−, 7 Cre+/−). Data analyzed by 2 way ANOVA. Data from APP/PS1/EKO mice (3 males, 1 female) are also plotted, but were not included in statistical analysis