Table 3 BSC studies models of proteopathic inclusion pathologies beyond Aβ and tau
From: Organotypic brain slice cultures to model neurodegenerative proteinopathies
Human Pathology | Slice Culture Pathology | Reference(s) |
|---|---|---|
α-synuclein inclusions | rAAV expression of WT or A53T human α-synuclein in BSCs induces pser129 α-synuclein inclusions. rAAV expression of A53T human α-synuclein in nigrostriatal circuit BSCs also develop α-synuclein inclusions. | |
SOD-1 inclusions | SOD-1 accumulation in spinal cord slice cultures from transgenic G93A SOD-1 mice from 3 weeks in culture. SOD-1 inclusions also inducible in G85R SOD-1 transgenic spinal cord cultures with human SOD-1 seeds from spinal tissues progressively over a 20 day incubation. | |
TDP-43 inclusions | BSCs from rats expressing human mutant TDP-43 develop TDP-43 inclusions and associated micro- and astrogliosis from 10 DIV. | [87] |
Huntingtin inclusions | Biolistic transfection of human wild-type and mutant huntingtin (HTT) in striatal and cortical rat BSCs results in the development of HTT inclusions by 7 DIV. | [89] |