Table 2 Antibodies used for western blot and immunocyhistochemistry
From: Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway
Antibody | Source | Dilution |
|---|---|---|
α-Synuclein (mouse) | BD Transduction Laboratories (61078) | 1:2000 (WB) |
α-Synuclein (mouse) | Biolegend (SIG39730) | 1:2000 (WB, ICC) |
α-Synuclein, clone 5G4 (mouse) | Millipore (MABN389) | 1:1000 (WB) |
Oligomer A11 (rabbit) | Thermo Fisher Scientific (AHB0052) | 1:1000 (WB) |
SIRT3 (mouse) | Santa Cruz (sc-135,796) | 1:1000 (WB) |
SIRT3 (rabbit) | Cell Signaling (2627 s) | 1:2000 (WB) |
SIRT3 (rabbit) | Novus Biologicals (NBP1–31029) | 1:1000 (WB) |
| Â | Â | 1:500 (ICC) |
SIRT3 (rabbit) | Cell Signaling (5490) | 1:1000 (WB) |
Heme oxygenase-1 (rabbit) | Cell Signaling (5853 s) | 1:1000 (WB) |
AMPK (rabbit) | Cell Signaling (5831 T) | 1:1000 (WB) |
Phospho-AMPK (rabbit) | Cell Signaling (2535 s) | 1:1000 (WB) |
CREB (rabbit) | Cell Signaling (4820 s) | 1:1000 (WB) |
Phospho-CREB (rabbit) | EMD Millipore (06–519) | 1:2000 (WB) |
DRP1 (rabbit) | Bethyl Laboratories (A303-410A-M) | 1:2000 (WB) |
Phospho-DRP1 (rabbit) | Cell Signaling (3455 s) | 1:1000 (WB) |
SOD2 (rabbit) | abcam (ab13533) | 1:5000 (WB) |
SOD2 (acetyl K68) (rabbit) | abcam (ab137037) | 1:2000 (WB) |
COX IV (rabbit) | Cell Signaling (4850 s) | 1:1000 (WB) |
GAPDH (rabbit) | Santa Cruz (sc-25,778) | 1:4000 (WB) |
| Â | Abgent (AP7873a) | 1:4000 (WB) |
Actin (mouse) | Sigma (A5316) | 1:7500 (WB) |
GM130 (rabbit) | abcam (ab52649) | 1:2000 (WB) |
Alexa Fluor 488 (goat anti-mouse) | Thermo Fisher Scientific (A11001) | 1:500 (ICC) |
Alexa Fluor 568 (goat anti-rabbit) | Thermo Fisher Scientific (A11011) | 1:500 (ICC) |
Goat anti-mouse HRP | Southern biotech (1010–05) | 1:5000 (WB) |
Goat anti-rabbit HRP | Southern biotech (4010–05) | 1:5000 (WB) |
IRDye® 680RD Goat anti-Mouse IgG (H + L) | LI-COR® (926–68,070) | 1:10000 (WB) |
IRDye® 800CW Goat anti-Mouse IgG (H + L), 0.5 mg | LI-COR® (926–32,210) | 1:10000 (WB) |
IRDye® 800CW Goat anti-Rabbit IgG (H + L), 0.5 mg | LI-COR® (926–32,211) | 1:10000 (WB) |