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Table 2 Antibodies used for western blot and immunocyhistochemistry

From: Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway

AntibodySourceDilution
α-Synuclein (mouse)BD Transduction Laboratories (61078)1:2000 (WB)
α-Synuclein (mouse)Biolegend (SIG39730)1:2000 (WB, ICC)
α-Synuclein, clone 5G4 (mouse)Millipore (MABN389)1:1000 (WB)
Oligomer A11 (rabbit)Thermo Fisher Scientific (AHB0052)1:1000 (WB)
SIRT3 (mouse)Santa Cruz (sc-135,796)1:1000 (WB)
SIRT3 (rabbit)Cell Signaling (2627 s)1:2000 (WB)
SIRT3 (rabbit)Novus Biologicals (NBP1–31029)1:1000 (WB)
  1:500 (ICC)
SIRT3 (rabbit)Cell Signaling (5490)1:1000 (WB)
Heme oxygenase-1 (rabbit)Cell Signaling (5853 s)1:1000 (WB)
AMPK (rabbit)Cell Signaling (5831 T)1:1000 (WB)
Phospho-AMPK (rabbit)Cell Signaling (2535 s)1:1000 (WB)
CREB (rabbit)Cell Signaling (4820 s)1:1000 (WB)
Phospho-CREB (rabbit)EMD Millipore (06–519)1:2000 (WB)
DRP1 (rabbit)Bethyl Laboratories (A303-410A-M)1:2000 (WB)
Phospho-DRP1 (rabbit)Cell Signaling (3455 s)1:1000 (WB)
SOD2 (rabbit)abcam (ab13533)1:5000 (WB)
SOD2 (acetyl K68) (rabbit)abcam (ab137037)1:2000 (WB)
COX IV (rabbit)Cell Signaling (4850 s)1:1000 (WB)
GAPDH (rabbit)Santa Cruz (sc-25,778)1:4000 (WB)
 Abgent (AP7873a)1:4000 (WB)
Actin (mouse)Sigma (A5316)1:7500 (WB)
GM130 (rabbit)abcam (ab52649)1:2000 (WB)
Alexa Fluor 488 (goat anti-mouse)Thermo Fisher Scientific (A11001)1:500 (ICC)
Alexa Fluor 568 (goat anti-rabbit)Thermo Fisher Scientific (A11011)1:500 (ICC)
Goat anti-mouse HRPSouthern biotech (1010–05)1:5000 (WB)
Goat anti-rabbit HRPSouthern biotech (4010–05)1:5000 (WB)
IRDye® 680RD Goat anti-Mouse IgG (H + L)LI-COR® (926–68,070)1:10000 (WB)
IRDye® 800CW Goat anti-Mouse IgG (H + L), 0.5 mgLI-COR® (926–32,210)1:10000 (WB)
IRDye® 800CW Goat anti-Rabbit IgG (H + L), 0.5 mgLI-COR® (926–32,211)1:10000 (WB)
  1. WB Western blot, ICC Immunocytochemistry