Fig. 5

Pharmacological chaperone affects synaptic integrity and neuroinflammation in 3xTg mice. a Representative western blot analysis of synaptophysin (SYP), and post-synaptic density protein-95 (PSD-95) in brain cortex homogenates from 3xTg mice treated with TPT (3xTg/TPT) or control (3xTg). b Densitometric analyses of the immunoreactivities presented in the previous panel. Values represent mean ± standard error of the mean (*p < 0.05, n = 4). c Representative images of brain hippocampus sections from mice treated with TPT (3xTg/TPT) or control (3xTg) immuno-stained with SYP antibody (scale bar: 100 μm). d Quantification of the integrated optical density (IOD) for the immunoreactivity to the same antibody shown in panel c. Values represent mean ± standard error of the mean (*p < 0.05, n = 4). e Representative Western blot analyses of glial fibrillary acidic protein (GFAP) and IBA1 in brain cortex homogenates from 3xTg mice treated with TPT or control (3xTg). f Densitometric analyses of the immunoreactivities presented in the previous panel. Values represent mean ± standard error of the mean (*p < 0.05, n = 4). g Representative images of brain cortex sections from mice treated with TPT (3xTg/TPT) or control (3xTg) immuno-stained with GFAP antibody (scale bar: 100 μm). (H) Quantification of the integrated optical density for the immunoreactivity to the same antibody shown in panel g. Values represent mean ± standard error of the mean (*p < 0.05, n = 4 per group)