Fig. 7

Lrrk2-deficiency caused nuclear hypertrophy and increased nuclear invaginations in SPNs after prolonged culture. a Co-staining of Lamin B and CTIP2 in Lrrk2^+/+ and Lrrk2^−/− SPNs after 3 weeks in culture. Scale bar, 10 μm. b, c The areas of SPN nuclei were measured from six independent Lrrk2^+/+ and Lrrk2^−/− cultures (b) and cumulative (Cum.) frequency was calculated to show the nuclear size distribution in each genotype (c). N = 300 neurons per genotype. Conditional logistic regression test, ^****p < 0.0001. d Ratio of SPN nuclei containing ≥1 invagination was calculated from three independent Lrrk2^+/+ and Lrrk2^−/− cultures. N = 200 neurons per genotype. Unpaired t-test, ^*p = 0.0181. e, f Sample EM images of cultured Lrrk2^+/+ and Lrrk2^−/− striatal neurons (e). The boxed area was shown in f. M indicates mitochondria. Scale bars: 2 μm (e), 0.5 μm (f). g-j The nuclear area (g), perimeter (h), and circularity (i), as well as the ratio of nuclei containing 0 to 4 invaginations (j) were calculated from the EM images. N = 7 and 15 neurons for Lrrk2^+/+ and Lrrk2^−/− cultures. Unpaired t-test, ^**p = 0.0018 (area), ^**p = 0.0045 (perimeter), ^***p = 0.0005 (circularity). k Co-staining of Lamin B and dsRed-Mito in a Lrrk2^−/− striatal neuron. Scale bar, 5 μm. l Co-staining of NUP98 and Lamin B in a Lrrk2^−/− striatal neuron. Scale bar, 5 μm