Fig. 7

Dopaminergic knockdown of Drosophila PARIS rescues dopaminergic neurotoxicity associated with reduced parkin or PINK1 activity. a Immunoblot analysis and quantification of dPARIS protein levels in flies expressing the indicated transgenes under the control of TH-Gal4 driver. b Dopaminergic knockdown of dPARIS promotes DA neuron survival under conditions of parkin or PINK1 knockdown in 30-day old flies. N = 10 flies per genotype. c Climbing defects associated with dopaminergic knockdown of parkin or PINK1 restored by dPARIS knockdown in DA neurons. N = 60 flies per indicated genotype. TH-Gal4/+ flies served as control. d Quantitative RT-PCR analysis of Drosophila homologs of PGC-1α (Spargel), NRF1 (ewg), NRF-2 (Delg) and mitochondrial transcriptional factor A (TFAM) in 30-day old fly heads from the indicated genotypes, N = 3. Quantitative data = mean ± SEM. One-way ANOVA *p < 0.05, **p < 0.01, *** p < 0.001, ****p < 0.0001. See also Additional file 16, Figure S6