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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency

Fig. 8

Induction of PTC readthrough in the mouse brain expressing R493X GRN-V5. a 6-week-old AAV-GRN-R493X-V5 mice were stereotactically injected ICV with either vehicle solution, G418, CDX5–288, or a combination of G418 + CDX5–288 and sacrificed after 72 h. Whole-brain RIPA-soluble protein extracts were subjected to automated capillary electrophoresis western analysis. Full-length PGRN was detected with a V5 antibody and actin was measured in brain lysates as a loading control. b Chemiluminescence quantification of automated capillary electrophoresis western analysis V5 detection normalized to actin. Values are shown as mean ± SEM; * p < 0.05 was determined by one-way ANOVA with Tukey’s multiple comparison test

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Molecular Neurodegeneration

ISSN: 1750-1326