Fig. 4

Mouse-to-human substitutions at positions 121 and 122 enhance the ability of m-aSyn to elicit dopaminergic fiber loss in rat striatum. Representative striatal rat brain sections stained for TH or VMAT2 (6–10 per group) are shown in panels a-e or f-j, respectively, as low-magnification images, and in panels a’-e’ or f’-j’, respectively, as corresponding high-magnification images. The imaged sections were from animals injected with the highest vector dose. The first panel of each row shows a representative image of the contralateral uninjected side (a, a’, f, f’). Quantified staining intensities of the striatum are displayed in k for TH and l for VMAT2. Data are presented as the percentage of corresponding contralateral staining intensity ± SEM. *p < 0.05; two-tailed Mann-Whitney U test. Scale bar, 1 mm (panels a-j) or 50 μm (panels a’-j’). See also Figure S2