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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Impaired NHEJ repair in amyotrophic lateral sclerosis is associated with TDP-43 mutations

Fig. 8

DNA damage correlates with TDP-43 pathology in a mouse model of ALS. DNA damage is evident in cortical neurons displaying cytoplasmic TDP-43 from rNLS mice at early stages of disease (1 week after doxycycline removal). Immunohistochemistry against (a) γH2AX, Scale bars 10 μM, white arrows indicate γH2AX foci (b) Quantification of images in (a) reveals significantly more cortical neurons with γH2AX foci in TDP-43 mice compared to non-transgenic aged and sex-matched controls, t-test, Mean ± SEM *p < 0.05. At least 180 neurons per mouse were counted, N = 3 (c) Immunoblotting reveals upregulation of γH2AX in cortical lysates from TDP-43 mice compared to controls, t-test *p < 0.05, Mean ± SEM, N = 3

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