Fig. 3

Prdx6 gene dose does not affect global response of astrocytes to Aβ deposition but shows a direct relationship with that of microglia. a, d, g Representative microphotographs of anti-GFAP, −Iba1, or -CD68 immunolabeled coronal sections through the somatosensory cortex from 10-month-old female mice of indicated genotypes, respectively. Counterstaining with Thioflavin-S (Th-S) reveals fibrillar plaques to which the load of glial cells was indexed. b, e, and h Quantitative analysis of GFAP⁺, Iba1⁺, and CD68⁺ cell load, in the brain cortex, respectively. c, f, and i Show loads of GFAP⁺, Iba1⁺, and CD68⁺ cells indexed to these of Th-S⁺ fibrillar plaques, respectively. While Th-S⁺-indexed GFAP⁺ cell load demonstrates no statistically significant differences across the genotypes, those for Iba⁺ and CD68⁺ cells have significant direct relationship with Prdx6 gene dosage. All values represent mean (+SEM) from n = 12 mice per genotype. p < 0.0001 in b, f, h, and i, p = 0.27 in c and p = 0.0142 in e (ANOVA); *p < 0.05, and ****p < 0.0001 (Holm-Sidak’s post-hoc test). Scale bar: 50 μm in a, d, and g