Fig. 9

Microglial/macrophage infiltration is most strong in the ipsilateral peri-infarct area of pMCAo+D mice. Microglial/macrophages were stained by Iba1 immunostaining. a Schematic representation of the infarct area at 2 levels of a coronal brain section and regions (red boxes) from which numbers of stained cells were estimated. c, cortex; CC, corpus callosum; V, ventricle; SVZ, subventricular zone. b Iba1 immunostaining in pMCAo + D mice, ramified Iba1+ cells in the peri-infarct area and amoeboid Iba1+ cells in the core of the infarct; scale bar: 100 μm. c In the peri-infarct area, microglia/macrophages density was significantly higher in pMCAo+D mice than in C, D (P < 0.0001) or pMCAo mice (P < 0.05). Cell density was higher in pMCAo mice than in C or D mice (P < 0.001). d No significant differences were evidenced in the contralateral mirror region of the infarct. e Iba1+ cell density was higher in ipsilateral striatum of pMCAo+D mice than for C mice and in pMCAo mice than for C mice (P < 0.05). f No significant differences in cell density were detected in contralateral striatum for the different animal groups.g No significant differences in cell density were evidenced in ipsilateral hippocampus. h Iba1+ cell density was higher in contralateral hippocampus of pMCAo mice than in C mice. P < 0.05, *P < 0.05, ***P < 0.001, ****P < 0.0001