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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Methods to investigate intrathecal adaptive immunity in neurodegeneration

Fig. 1

Cryopreservation of human cerebrospinal fluid cells retains cellular viability. a Workflow for cryopreservation of CSF cells for scRNA-TCRseq. b Gating strategy for sorting live cryopreserved cells by flow cytometry. c Quantification of live cell count (live singlets) from flow cytometry sorting following cell thawing. Mean ± s.e.m. d Quantification of viability (percentage of live singlets out of all singlets) from sorting by flow cytometry. Mean ± s.e.m. e Linear regression of live cell count post thaw versus length of storage. f Quantification of the number of CSF cells captured for sequencing

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Molecular Neurodegeneration

ISSN: 1750-1326