Fig. 3

Reduced dendritic spine density in hippocampal neurons of Clu KO mice. a Representative dendritic spines from Thy1-GFP-labeled dendrites in area CA1 of hippocampal pyramidal neurons of four-month-old littermate WT and Clu KO mouse brains. IMARIS was used to reconstruct the 3D rendering of the spines on dendrites based on confocal images of GFP-positive neurons and to classify the four spine types (mushroom, stubby, long-thin, and filopodia). Scale bar, 2 μm. b Total spine density in WT and Clu KO mouse brains. c Density of each of the four spine types on secondary apical dendrites of WT and Clu KO mouse brains. Dendritic segments were derived from 3 random neurons per mouse/ 4 animals for each group. All data are presented as mean ± SEM. *p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (Student’s t test)