Fig. 9
Abnormal oscillatory activities and the effect of TGB in the OB of 3xTg mice. a Representative gamma oscillation in the OB of 3–5 month-old WT and 3xTg mice. b-c Unchanged power in the theta and beta band of 3xTg compared to WT mice. d-e Significantly increased power in low-gamma band (p = 0.0007) and similar power in high-gamma band (p = 0.195) of WT and 3xTg mice. f TGB significantly reduced gamma power and normalized the difference of gamma oscillation between 3xTg and WT (n = 11 for WT and n = 13 for 3xTg; p = 0.0001, WT vs 3xTg; p = 0.0001, 3xTg vs 3xTg + TGB; p = 1, WT + TGB vs 3xTg + TGB). g-h Representative immunoblots and quantification of excitation- and inhibition-related protein levels in the OB (n = 3, per genotype, two repeats, GluR1: p = 0.032; GABAARβ2: p = 0.026; GABAARβ3: p = 0.001; GABAARα1: p = 0.02). (i-l) Representative images and statistical analysis of immunofluorescent staining of GABAAR α1 and β3 subunits in the OB of WT and 3xTg mice at 3–5 months (n = 3–4 sections/mouse, 3 mice for WT and 5 mice for 3xTg; GABAAR α1: p = 0.036 for MCL, p = 0.032 for EPL, p = 0.021 for GL; GABAAR β3: p = 0.021 for MCL, p = 0.019 for EPL, p = 0.033 for GL). Values represent mean ± SEM. Two-sample t-test. *p < 0.05; **p < 0.01; *** p < 0.001