Fig. 6

Pyramidal neurons in the hippocampus from AD patients display OPTN in HDA6C-positive aggregates associated with high mitochondrial cholesterol levels. a and b Hippocampal slices from control (CTRL) and AD patients classified into three groups following the “ABC” score: CTRL, intermediate AD (AD III-IV) and high AD (AD VI). Shown are representative confocal photomicrograph of double immunofluorescence for OPTN (green) and HDAC6 (red) at 20X magnification (a) and 63X magnification (b). A negative control slide incubated only with secondary antibodies was included. c Shown are representative confocal photomicrograph of double immunofluorescence for GST-PFO (green) and TOMM20 (red). Sections were incubated with 20 μg/ml GST-PFO for 3 h prior immunolabeling. Insets show a 3-fold magnification of the indicated regions. A negative control without GST-PFO incubation was included. Graphs depict the intensity profiles of GST-PFO and TOMM20 fluorescence in the cross-section. Nuclei were counterstained by DRAQ5 (blue). Scale bar: 50 μm (a) and 25 μm (b and c). In all the cases, the Pearson’s correlation coefficient (PCC) was calculated from 5 individuals per group (at least 6 random fields were analyzed per sample). *P < 0.05; **P < 0.01 (data are mean ± SD)