Fig. 4

High or low dose of control AAV-GFP virus does not affect retinal structure and function in adult albino mice. a GFP immunostaining in retinas from 2-month-old albino mice 2 weeks after infection with a low or a high dose of AAV-GFP. b Western blotting of GFP in retinas from albino mice under the indicated conditions. c Quantification of GFP expression in retinas according to the above immunoblots (Error bars: SD; n = 3, one-way ANOVA). d ERG scotopic traces of rod response and standard response from albino mice 2 weeks after infection with low or high dose of AAV8-GFP virus. e Quantification of the a- and b-wave amplitudes according to ERG traces of the standard response (Error bars: SD; n = 6, one-way ANOVA). f Retinal structure of albino mice 2 weeks after infection with a low or high dose of AAV8-GFP virus. g Quantification of the thickness of the photoreceptor cell layer according to results of the retinal structure shown in (f) (Error bars: SD; n = 6, one-way ANOVA). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; PL, photoreceptor layer; ns, no significant difference; * or ** or *** indicates p < 0.05 or p < 0.01 or p < 0.001. Scale bars: 50 μm