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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: The CD33 short isoform is a gain-of-function variant that enhances Aβ1–42 phagocytosis in microglia

Fig. 2

Differential regulation of phagocytosis by hCD33M and hCD33m in U937 cells. a Flow cytometric analysis of phagocytosis of aggregated fluorescent Aβ1–42 in hCD33+/+ and hCD33−/− U937 clones. Levels of phagocytosis are standardized to the average of the three CD33+/+ set to 100%. Each data point represents one clone, which is itself the average of three replicates. Clones C4 and A6 are highlighted as they were used in subsequent transduction studies (N = 3 CD33+/+; N = 11 CD33−/−). % Phagocytosis represents the cytochalasin-D subtracted values referenced to the average of the CD33+/+ cells set to 100%. (N = 6) b,c hCD33M and hCD33m were transduced into CD33−/− U937 cells (b) clone C4 and (c) clone A6, along with an empty virus control. Phagocytosis was assessed with aggregated fluorescent Aβ1–42 phagocytosis by flow cytometry. % Phagocytosis represents the cytochalasin-D subtracted values referenced to the average of the empty vector (EV) cells set to 100%. (B, N = 5 replicates; C, N = 3 replicates)

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