Fig. 7

Os-pep via AdipoR1 dependently improved the learning and memory of AD, Adipo^−/− and AdipoR1 shRNA mice. MWM and Y-maze assays were used to assess the spatial working memory and cognitive functions in the AD mouse models and in the Adipo^−/− mice. Thirteen mice from each group were used for the behavioral evaluation. (a-c) The mean escape latency (sec) to reach the hidden platform during training of the AβO-treated, APP/PS1 and Adipo^−/− mice. (d-i) The time spent in the target quadrant and number of platform crossings during the probe test of the AβO-treated, APP/PS1 and Adipo^−/− mice. (j-l) Histograms present the percentage of spontaneous alternation behaviors by the AβO-treated, APP/PS1 and Adipo^−/− mice respectively during the Y-maze test. (m) The mean escape latency (sec) to reach the hidden platform during training of the AβO-treated (subjected to scramble and functional AdipoR1 shRNA). (n, o) The time spent in the target quadrant and number of platform crossings during the probe test of the AβO-treated (subjected to scramble and functional AdipoR1 shRNA). (p) Histograms present the percentage of spontaneous alternation behaviors by the AβO-treated (subjected to scramble and functional AdipoR1 shRNA) during the Y-maze test. The escape latencies in the behavioral tests were analyzed using a two-way ANOVA, with training days as the repeated measurements. Graphs show the means ± SEM for the mice (13 mice per group) and the number of independent experiments = 3. Significance = *p < 0.05, **p < 0.01 ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001; One-way ANOVA followed by Turkey’s post hoc test. ns, indicated that there is no significant difference among the control, sham and Os-pep treated groups