Fig. 1

Hyperacetylation of multiple histone sites in the PD prefrontal cortex. Representative Western blots and the ratios of their quantified densitometric measurements of the indicated proteins (or their modifications) in individuals with PD (orange) and controls (blue) are shown. GAPDH served as loading control. Values were rescaled [0,1]. Boxplots show the mean of replicates for each individual. The indicated p-values are based on a linear mixed model (see Methods). a Global protein acetylation detected by a pan-acetyl lysine antibody. b Relative acetylation of histone lysine residues and quantification of the total histone proteins. c Relative acetylation of α-tubulin on lysine K40. d Immunodetection and densitometric quantification of relative protein levels of the nuclear marker (NUP188) and cell type-specific marker proteins: NeuN – neurons, GFAP – astrocytes, CNP1 – oligodendrocytes, CX3CR1 - microglia