Fig. 4
From: Oligomerization of Lrrk controls actin severing and α-synuclein neurotoxicity in vivo
Lrrk promotes mitochondrial dysfunction and Drp1 mislocalization in α-synuclein transgenic flies. a-d, Metabolic profiling of whole brains in Seahorse XF 96-well culture microplates (Agilent) reveals decreased oxygen consumption rate (a, OCR), mitochondrial ATP production (b) and basal respiration (c) mediated by a-synuclein expression, which is worsened by Lrrk manipulation when compared to flies expressing α-synuclein alone. d, OCR and extracellular acidification rate (ECAR) from the same experiment plotted simultaneously reveal overall metabolic profiles for each genotype. n=4-9 per genotype. Data are represented as mean ± SD. *p<0.05, **p<0.01, ***p<0.005, ANOVA with Bonferroni post-test analysis. Control is nSyb-GAL4, nSybQF2/+. Flies are 10 days old