Fig. 2

Morphological and Physiological Characterization of αON-Sustained RGCs in WT and Wld^S+/+ Control Retinas. A Example en face (top) and orthogonal (bottom) images of dye-filled (AL555, cyan) αON-S RGCs from WT and Wld^S+/+ Ctrl animals. αON-S RGCs were identified by positive immunolabeling against SMI-32 (magenta) and dendritic projections within the ON sublamina of the IPL identified by ChAT (red) immunolabeling. B Average light-evoked (3 s 365 nm, horizontal dashed line) spike rate histograms (300 ms bin width) of αON-S RGCs from WT and Wld^S+/+Ctrl eyes. C Wld^S+/+ Ctrl αON-S RGC light responses are more robust than WTs (*, p ≤ 0.008), D but RMP of WT and Wld^S+/+ Ctrl αON-S RGCs is similar (p = 0.47). E Sholl analysis of WT and Wld^S+/+ Ctrl αON-S RGCs. F Dendritic branch points, field area, and length are similar between WT and Wld^S+/+ Ctrl αON-S RGCs (p ≥ 0.23), but Wld^S+/+ somas are significantly larger (p = 0.01). WT Ctrl group contained cells from WT saline (n = 59) and WT naïve (n = 14) eyes. Wld^S+/+ Ctrl group consisted of cells from Wld^S+/+ saline-injected (n = 18) and naïve (n = 15) eyes. Statistics: Mann-Whitney tests ( C, D, F, Field Area, Soma Size); Two-Way Repeated Measures ANOVA, Bonferroni post hoc tests (E), Student’s t-tests (F, branch points and length). Scale bars = 40 μm. Arrows indicate RGC axons. Data = mean ± SEM