Fig. 7
Alteration in protein levels of DN-forming proteins in APPNL-G-F mouse brains and in vitro Aβ42 treated cells/neurons. A Protein lysates from the cortex of 3-month (3 m) and 9-month (9 m) old WT and APPNL-G-F mice were prepared for Western blot analysis with antibodies to LAMP1, PSAP, SAP-C, SAPs, ATG9A and calnexin. B Quantification for each protein band was conducted using Fiji software and the average of each age group was normalized to the calnexin level. C Lysates prepared from N2a cells treated with vehicle only (0) or Aβ42 (0.5 to 4 μM) for 16 h (h) were subjected to Western blot analysis. D Band intensity for each protein was measured and normalized to the intensity of β-actin. E, F Primary neurons, cultured form the cortical and hippocampal regions, were also treated with vehicle or 1 μM Aβ42 for 16 h (h), and protein lysates were examined by Western blot analysis (E) and similar quantification (F). N = 3 independent experiments in all western analyses (* P < 0.05, ** P < 0.01, student t-test)