Fig. 2
From: SETD7-mediated monomethylation is enriched on soluble Tau in Alzheimer’s disease
Levels of meK130 and meK132-modified Tau increase with Braak stage in humans and with age in rTg4510 mice in the soluble fraction. A Total Tau levels decrease while methylated Tau levels increase with Braak stage in the soluble fraction (S1) of human brain. B Total and methylated Tau levels increase in parallel at high Braak stages in the sarkosyl-insoluble fraction (P3). C Electrochemiluminescence ELISA demonstrates an age-dependent increase in meK130 and meK132-modified Tau in Triton-X soluble fractions of rTg4510 mouse hippocampus. Data are normalized to total Tau levels obtained by Tau 5 ELISA. A-C Statistical significance was determined by one-way ANOVA (S1 total Tau: ANOVA p < 0.001, S1 meK130: ANOVA p < 0.0001, S1 meK132: ANOVA p < 0.001; P3 total Tau: ANOVA p < 0.05, P3 meK130: ANOVA p < 0.0001, P3 meK132: ANOVA p < 0.001, rTg4510 meK130: ANOVA p = 0.0002, rTg4510 meK132: ANOVA p < 0.0001). Tukey’s multiple comparisons test was used for post-hoc analysis. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001