Fig. 1
From: Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis
Plasma EVs are efficiently purified by NBI. a Schematics of the purification strategy and validation. b Immunoblot for flotillin-1, syntenin, CD81 (markers of EVs) and GM130, calnexin and cytochrome C (contaminants of EVs) in plasma EV samples (EV1, EV2, EV3) isolated by NBI, and EV-depleted plasma (Surn1, Surn2, Surn3). Loading control represented by Red Ponceau. c TEM analysis of the plasma EV purified with NBI: one representative image from three independent experiments. Bar, 500 nm in the main panel; 200 nm, 100 nm, 200 nm in the insets from top to bottom