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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Processing of progranulin into granulins involves multiple lysosomal proteases and is affected in frontotemporal lobar degeneration

Fig. 2

PGRN undergoes pH-dependent processing into multi-granulin fragments and individual granulins in vitro. a, Schematic representation of the antibodies used. Anti-paragranulin (p-Gran) in green, anti- granulin F (Gran-F) in red and anti-granulin E (Gran-E) in blue. b-d, For in vitro protease assays, 400 ng of PGRN was incubated for 20 min with or without 1 μM of each enzyme at the indicated pH. Western blotting analysis was performed using antibodies as indicated. pH-dependent cleavage products are indicated with red asterisks (*). Progranulin (PGRN), multi-granulin fragments (MGFs), cathepsin B (CTSB), cathepsin L (CTSL), cathepsin K (CTSK), cathepsin S (CTSS), asparagine endopeptidase (AEP), cathepsin V (CTSV), cathepsin G (CTSG), cathepsin E (CTSE). A representative of n = 3 replicates is shown

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Molecular Neurodegeneration

ISSN: 1750-1326