Fig. 6

L-DL regulates cytoskeleton- and synapse-related gene expressions via modulating their alternative splicing. a Cellular component terms for genes with changed alternative splicing in the hippocampus of L-DL knockdown (KD) mice in GO analysis (n = 1,631, P < 0.001). b Diagram for cellular localization of synapse-, dendrite- and cytoskeleton-related proteins, with their genes undergoing  aberrant alternative splicing in neurons. c, d Alternative splicing products of CADM1, GIT2, CHL1, CASK, AGRIN, GIT1, CTTN, PSD95, ADGRL1, SYNJ1, EPB41L3 and MAPT gene, determined by RT-PCR in the hippocampus of control and L-DL KD mice (n = 3). * indicates spliced variants, F and R denote primers used for RT-PCR. 24 cycles or 30 cycles were used to detect higher abundant and lower abundant splicing products, respectively. e, f Alternative splicing products of CAMKV determined by RT-PCR (e) and densitometric analyses (f) in the hippocampus of control and L-DL KD mice (n = 3). I4, intron 4 retention; ΔI4, intron 4 exclusion. g, h Protein levels of CAMKV, SNAP25, PSD95, NR2B in the hippocampus of control and L-DL KD mice, measured by immunoblotting (g) and densitometric analyses (h). GAPDH was included as an input control. Statistical analysis was performed using two-tailed Student’s t-test; * P < 0.05, ** P < 0.01, *** P < 0.001; error bars denote the SEM