From: Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model
Key Resources Table | ||
Antibodies | Source | Identifier |
pSer129 αSyn | B. Giasson University of Florida College of Medicine; Florida; USA | 81A [48] |
2–21 mouse and human αSyn | B. Giasson University of Florida College of Medicine; Florida; USA | 2H6 [49] |
130–140 mouse and human αSyn | B. Giasson University of Florida College of Medicine; Florida; USA | 94-3A10 [49] |
120–125 human αSyn | B. Giasson University of Florida College of Medicine; Florida; USA | |
pSer129 αSyn | Abcam | EP1536Y [51] |
1–16 Αβ | T. Golde University of Florida College of Medicine; Florida; USA | Ab5 [52] |
4–10 Αβ; APP | Biolegend | 6E10; Cat # 803003 |
x-42 Αβ specific | EMD Millipore Corporation, Temecula, CA | 12F4; Lot: 3270770 |
x-40 Α specific | T. Golde University of Florida College of Medicine; Florida; USA | 13.1.1 [52] |
1–16 Αβ | T. Golde University of Florida College of Medicine; Florida; USA | 33.1.1 [52] |
GFAP | Dako, Carpentaria, CA | GFAP Dako Cat # Z0334 |
p62/sequestresome1 | ProteinTech, Rosemont, IL | |
NFL | Cell Signaling | Cat# C28E10 |
5G4 | Fisher Scientific | Cat # MABN389MI [53] |
Actin | Fisher Scientific | Clone C4; Cat# MAB1501MI |
Experimental Models: Organisms/Strains | ||
Cohort Name | Source | N |
Line 85 | D. Borchelt University of Florida College of Medicine; Florida; USA | M:12; F:14 |
M20 | B. Giasson University of Florida College of Medicine; Florida; USA | M:12; F:14 |
dTg | D. Borchelt University of Florida College of Medicine; Florida; USA | M:11; F:14 |
C3H/BL6 (nTg) | Charles River | M:13; F:16 |
Chemicals, peptides, and Recombinant Proteins | ||
Human α-synuclein PFFs | This manuscript | N/A |
Software and Algorithms | ||
Prism 7 | GraphPad |