Fig. 8

Myelin sulfatide deficiency resulted in upregulation of SPI1, STAT3, and C/EBPβ transcription factors in CNS. (A) The transcription factors (TF) score by using ChEA3 (ChIP-X Enrichment Analysis Version) with 72 DEGs listed in Fig. 3C. (B) Western blot analysis from cerebrum and spinal cord of 9 mo post-injection CST cKO mice using antibodies against transcription factors SPI1, C/EBPβ, IRF8, Smad2/3, STAT3 and its Y705 phosphorylation. (C) Relative expression was quantified and plotted as a ratio to GAPDH or β-actin for (B). (D) Western blot analysis from cerebrum of CST^+/+ and CST^−/− mice with or without PLX3397 treatment using antibodies against STAT3 and its Y705 phosphorylation and β-actin. (E) Relative expression was quantified and plotted as a ratio to β-actin for (D). (C,E) Two-tailed unpaired t-Test, n = 3. *p < 0.05, **p < 0.01. Data represent the mean ± S.E.M