Fig. 1
Evaluation of αSyn_RT-QuIC seeding activity triggered by the OM of patients with PD, MSA-P, MSA-C, and HS. RT-QuIC seeding activity of αSynD was observed in 9/13 OM samples of the same PD patients (a and b) and 18/20 of the same MSA-P patients (c and d) analyzed at ITA-lab (a and c) and USA-lab (b and d). None of the samples collected from MSA-C (e) or HS (g) induced a seeding activity at ITA-lab, while 1/10 OM sample collected from MSA-C patients (f) and 1/11 sample collected from HS (h) induced a seeding activity at USA-lab. Samples were analyzed at least three times in quadruplicate. Curves represented in the graphs were obtained by plotting the average fluorescence intensities of each sample against time. The brain homogenates (BH) of PD (dashed curves in a and b), MSA-P (dashed curves in c and d) and MSA-C (dashed curves in e and f) patients were diluted until 10− 4 and used as positive controls. The BH of a patient not affected by α-synucleinopathy (dashed curves in g and h) was diluted until 10− 4 and used as negative control