Fig. 3

The aggregated SnG network and cell-type enrichment. a The aggregated network from 125 SnG-enriched modules. In the network plot, the node corresponds to a gene from SnG-enriched modules, and the link corresponds to the co-expression relationship between genes in MEGENA co-expression networks. The aggregated network was generated by merging co-expression links between SnG-enriched module genes conserved in at least five tissues (Methods). The node size in the network plot is proportional to the node conservation in different tissues, and the gene symbols of the CellAge SnGs are labeled. b Comparison of the aggregated network with four SnG annotation datasets. A bar chart shows possible intersections among the five annotation datasets (including the aggregated network) in a matrix layout, with the solid and empty circles indicating the presence and absence of the gene sets for each intersection, respectively. The numbers to the right of the matrix indicate set sizes. The color bars on the top of the matrix show the intersection sizes. The color intensity is proportional to the one-tailed hypergeometric p-value significance. c Barplot showing the top 10 cell types whose marker genes are significantly enriched (FDR < 0.01) for the aggregated network genes. The FDR was calculated as the adjusted FET p-value. d The heatmap plot showing the cell types whose marker genes are enriched (FDR < 0.01) in the SnG-enriched modules. Each row shows the cell types, and each column shows the network module. The colors are scaled with the enrichment value. The barplot on the right shows the total frequency of cell-type enrichment