Fig. 4

SnG-enriched modules and associated cell types in the brain. a Sunburst plot of the SnG-enriched modules in the hippocampus. Each cell showed a hierarchical module from the co-expression network. The color intensity of each cell is proportional to the corresponding enrichment of the CellAge SnGs (FET p-value). b Sunburst plot of module correlation with donor age. For each network module, the Spearman correlation was calculated between the module eigengene and the donor age. The color intensity of each cell is proportional to the corresponding correlation p-value. c The enrichment of cell-type marker genes for the modules M81 and M82. For each cell type, the marker genes were identified from the snRNA-seq dataset by the Wilcoxon Rank Sum test. FDR was calculated as the adjusted FET p-value, which tested marker gene enrichment for M81 and the M82. d The co-expression network of M81. The hub genes in the module are labeled and shaded with red color. e The co-expression network of M82. The CellAge SnGs are labeled and highlighted with red color. The orange nodes are the hub genes. The node sizes are proportional to the connectivity in the network. f Violin plot of the SnGs preferentially expressed in VSMCs (top two rows) and endothelial cells (bottom two rows) in the brain snRNA-seq dataset. The x-axis shows the cell types in the scRNA-seq, and the y-axis shows the log-transformed normalized gene expressions