Fig. 7
Quantitative comparison of mPFC-CoA connections between Alzheimer’s disease and control mice by tracing with H129-dgK-G4. A. The tracing timeline. Helper virus AAV2/9-mCh-gK (1.0 × 1012 vg/ml, 150 nl) and H129-dgK-G4 (5.0 × 108 pfu/ml, 150 nl) were sequentially injected into the medial prefrontal cortex (mPFC) of wildtype C57BL/6 mice and 3 × Tg-AD mice at day 1 and day 22, and the brains were perfused and collected at day 27. CoA, cortical amygdaloid nucleus. B. Representative tracing results. Representative images of the labeled neurons in CoA (indicated with the dashed boxes) of the wildtype C57BL/6 and 3 × Tg-AD mice are shown. ACo, anterior cortical amygdaloid nucleus; PMCo, posteromedial cortical amygdaloid nucleus; PLCo, posterolateral cortical amygdaloid nucleus. C–E. Quantitative analysis. The numbers of mCherry/GFP double-labeled starter neurons in mPFC (C) and the GFP-labeled postsynaptic neurons in CoA (including Aco, PMCo, and PLCo in both hemispheres) (D) were counted in three mice for each group and shown. The statistical significance was analyzed by LME. **, p < 0.01. The average ratio of GFP+ CoA neurons to mPFC starter neurons was calculated and shown, and the statistical significance was analyzed by Student’s t-test. **, p < 0.01 (E)