Fig. 3
Dysregulation of microglial activation in the absence of Cx3cr1. (A) Volcano plots showing significant differentially expressed genes (DEGs) identified based on the Nanostring neuroinflammation panel to analyze cortical lysates from (i) 6 month-old female and (ii) 6 month-old male 5xFAD;Cx3cr1−/− mice when compared to sex-matched 5xFAD;Cx3cr1+/+ animals. (B-D) Quantitative real-time PCR (qRT-PCR) analyses performed on CD11b+ microglia purified from brains of 6 month-old 5xFAD;Cx3cr1+/+ and 5xFAD;Cx3cr1−/− mice to validate top DEGs from Nanostring analysis. Gene-expression associated with (Bi-Bv) Disease-associated microglial activation, (Ci-Cv) inflammatory activation and (Di-Diii) oxidative stress responses analyzed using purified microglia from 5xFAD mice with and without Cx3cr1. All gene-expression data are represented as fold-change compared to microglia isolated from female 5xFAD;Cx3cr1+/+ mice (black bars). Data in B-D represent mean ddCT values using 8 mice (4 females, 4 males) per genotype. Error bars represent SEM. Statistics done One-way ANOVA followed by Brown-Forsythe and Welch post-hoc tests. *padj < 0.05, **padj < 0.001, ***padj < 0.0001, ****p.adj < 0.00001